The influence of fasting and nitrogen deprivation on the concentration of free amino acids in rat plasma.
نویسندگان
چکیده
The microbiological assay procedure for the determination of amino acids is particularly well suited for routine analyses of low potency materials. Its application to metabolic studies includes the measurement of “apparent free” amino acids in plasma of a number of species (l-4), “free” (5-10) and total (6-10) amino acids in urine, and free amino acids in sweat (11) and cerebrospinal fluid (12). The use of these methods for such materials which may contain amino acids in combined forms, without preliminary hydrolysis, has been criticized. Some peptides can replace one or more of their constituent amino acids for lactic acid bacteria usually employed in assays (13-15). Nevertheless, such methods seem useful for studies in which comparative results are desired. They might conceivably become a useful tool for laboratory detection of pathological conditions. Schweigert et al. (2) and Dunn et al. (1) have employed these procedures for the determination of the tryptophan content of rat plasma and of tungstic acid filtrates of human blood fractions, respectively. Hier and Bergeim (3, 4), applying these procedures to tungstic acid filtrates, have determined the normal fasting plasma values for ten amino acids for man and the dog. Hier (16) studied the effect of administration of large doses of a number of single amino acids on the plasma levels of twelve amino acids. These workers (3) showed that acceptable recovery values were obtained, that the values were reproducible, and that the results were not affected by nembutal anesthesia, by prolonged contact of the protein-free plasma with the precipitate, or by long storage of the filtrate at low temperature.
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 177 2 شماره
صفحات -
تاریخ انتشار 1949